Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector. pSIP409: aiiA construct was stably maintained in L. plantarum NC8.

2014-08-22

For the. preparation of a standard for Akkermansia muciniphila, a. clone obtained from mice Probi Mage® bygger på mjölksyrabakterien Lactobacillus plantarum 299v (LP299V®). Denna bakterie är ett resultat av drygt tjugo års svensk forskning och är >W1710817704 · LKLZ01000009, Firmicutes, Lactobacillus plantarum WJL (LKLZ) Firmicutes, Lactobacillus plantarum subsp.

Lactobacillus plantarum nc8

Oxdc gene from Bacillus Lactobacillus plantarum NC8d d Kindly provided by Lars Axelsson from MATFORSK, Norwegian Food Research Institute, Osloveien, Norway. Inducible plantaricins (PLNC8 αβ, PlnEF and PlnJK) producer Conditional (Bac c c Ability to induce bacteriocin production in co‐culture with L. plantarum NC8. Influenza A virus (H1N1) is an acute, highly contagious respiratory virus. The use of lactic acid bacteria (LAB) to deliver mucosal vaccines against influenza virus infection is a research hot spot. In this study, two recombinant Lactobacillus plantarum strains expressing hemagglutinin (HA) alone or coexpressing aCD11c-HA to target HA protein to dendritic cells (DCs) by fusion to an anti-CD11c The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery.

Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316, has been shown to be highly potent against a range of bacteria and fungi.

2. 3. Jun 22, 2017 Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice.

Lactobacillus plantarum is a versatile bacterium found in a variety of ecological niches, ranging from vegetable and plant fermentations to the human gastrointestinal tract. L. plantarum cells are rods with rounded ends, straight, generally 0.9–1.2 μm wide and 3–8 μm long, occurring singly, in pairs or in short chains (Figure 43.1).

Unreviewed-Annotation score: -Protein predicted i.

Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730). J. Bacteriol. 194, 2391–2392 (2012). 1 Coculture with Specific Bacteria Enhances Survival of Lactobacillus plantarum NC8, an 2 Autoinducer-Regulated Bacteriocin Producer, in Olive Fermentations. 3 4 José Luis Ruiz-Barba*, Belén Caballero-Guerrero, Antonio Maldonado-Barragán, and Rufino 5 Jiménez-Díaz.
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3 Lactobacillus plantarum NC8 was shown to produce plantaricin NC8 (PLNC8), a recently 4 purified and genetically characterized inducible class IIb bacteriocin, only when it was co-5 cultured with other Gram-positive bacteria. Among 82 strains belonging to the genera Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector.

Mnemonic i-Taxon identifier i: 1036177: Scientific name i: Lactobacillus plantarum subsp. plantarum NC8: Taxonomy navigation 2020-01-22 2020-12-10 Lactiplantibacillus plantarum (previously Lactobacillus plantarum) is a widespread member of the genus Lactiplantibacillus and commonly found in many fermented food products as well as anaerobic plant matter.
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Lactobacillus plantarum is a versatile bacterium found in a variety of ecological niches, ranging from vegetable and plant fermentations to the human gastrointestinal tract. L. plantarum cells are rods with rounded ends, straight, generally 0.9–1.2 μm wide and 3–8 μm long, occurring singly, in pairs or in short chains (Figure 43.1).

Maldonado, J. L. 2020-12-10 · Axelsson, L. et al. Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730). J. Bacteriol. 194, 2391–2392 (2012). 1 Coculture with Specific Bacteria Enhances Survival of Lactobacillus plantarum NC8, an 2 Autoinducer-Regulated Bacteriocin Producer, in Olive Fermentations. 3 4 José Luis Ruiz-Barba*, Belén Caballero-Guerrero, Antonio Maldonado-Barragán, and Rufino 5 Jiménez-Díaz. Transformation of Lb. sakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475 with plasmids pSIP409UAI, pSIP411UAI and pMGUAI yielded recombinant Lactobacillus spp.-derived strains which were further checked by bacteriocinogenicity tests, PCR and sequencing of the inserts.

To construct the recombinant Lactobacillus plantarum NC8for expression of HA-DCpep fusion gene and to detect the immunogenicity of fusion protein,the HA-DCpep gene was amplified by PCR and cloned into pGEM-T vector.The target gene was cloned into Escherichia coli-Lactobacillus shuttle expression vector pSIP409after sequencing.The recombinant plasmid was electroporated into L.plantarum NC8,and

Bakteriociner är mycket Den antimikrobiella substansen PLNC8 αβ, en så kallad bakteriocin utsöndras från Lactobacillus plantarum NC8, vilket är en nyttiga Orsaken till det är troligtvis att stammar av L. acidophilus, vilken är bland de bulgaricus, Lactobacillus paracasei, Lactobacillus plantarum. Till exempel tillhör en utav bakteriestammarna i vårt kosttillskott Synbiotic släktet Lactobacillus, arten plantarum och stammen 2362.

In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. 5 Lactobacillus plantarum NC8, has been recently purified and genetically characterized. 6 Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the 7 presence of at least four operons involved in bacteriocin production, showing high 8 homology to the plantaricin cluster in L. plantarum C11. However, we found a three- Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp. Oxdc gene from Bacillus subtilis was polymerase chain reaction-ampliﬁed and Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many laboratories worldwide.